ABSTRACT
Objective To investigate the effects of polysaccharide sulfate(PSS)on lipopolysaccharide(LPS)-induced mice pulmonary microvascular endothelial cell(PMVEC) injury in vitro .Methods PMVEC were divided into the blank control group (C) ,LPS stimulation group (L) and PSS+ LPS group (LP) .The effect of PSS on the viability of LPS-induced PMVEC was ob-served by MTT assay .The influence of polymorphonuclear(PMN) on the PMVEC adhesion number was measured by the rose Ben-gal staining .The concentrations of TNF-αand ICAM-1 in culture supernatant of PMVEC were detected by ELISA assay .Results PSS could inhibit the decrease of PMVEC viability caused by LPS(P=0 .001) ,compared with the group C ,the adhesion number of PMVEC and PMN in the group L was significantly increased(P=0 .000) ,the expression of TNF-αand ICAM-1 was significantly increased(P=0 .000);compared with the group L ,PSS pretreatment for 1 h could significantly decrease the LPS caused adhesion of PMVEC and PMN(P=0 .000) ,the expression of TNF-αand ICAM in the group LP was significantly decreased (P<0 .05) .Con-clusion PSS can inhibit LPS-induced PMVEC injury and adhesion of PMVEC and PMN ,its mechanism may be related with the de-crease of ICAM-1 and TNF-αexpression .
ABSTRACT
OBJECTIVE:To investigate the protective effects of adjunctive general anesthesia of dexmedetomidine on cardiac and cerebral ischemia-perfusion injury. METHODS:50 patients,ASA physical status Ⅰ-Ⅱ,undergoing selective tracheal intuba-tion,were randomly divided into dexmedetomidine group (group D) and control group (group C) with 25 cases in each group. The patients in group D received a loading dose of 1 μg/kg of dexmedetomidine by infusion pump before anaesthesia,10 min lat-er by a maintenance dose of 0.5 μg/(kg·h)till the end of surgery;whereas patients in group C received normal saline and same induction. HR,SBP and DBP of 2 groups were monitored at the beginning of operation,10 min after incision,30 min after inci-sion,at the end of operation. Blood samples of vena jugularis interna were taken to determine the plasma levels of cardiac tropo-ninⅠ(cTnⅠ) ,adrenaline and norepinephrine before anesthesia induction (T0),at the end of surgery (T1),6 h after surgery (T2),12 h after surgery(T3),24 h after surgery(T4),48 h after surgery(T5). RESULTS:There was no statistical significance in the levels of HR,SBP and DBP in group D between during operation and at the beginning of operation(P>0.05);those in-dex were significantly lower than those of group C at corresponding time points,with statistical significance(P<0.05). The lev-els of cTnⅠ in group D at T2-T5 were significantly lower than in group C,with statistical significance(P<0.05). The levels of adrenaline and norepinephrine at T1-T5 were significantly lower than group C,with statistical significance (P<0.05). CONCLU-SIONS:Adjunctive general anesthesia of dexmedetomidline can improve cardiac and cerebral ischemia-perfusion injury to certain extent.
ABSTRACT
Objective:To research the influence of thoracic epidural block on blood dynamics、neural endocrine hormone and the expression of AQP4 mRNA in the lung of rabbit with heart failure models.Methods:60 rabbits which were punctured and cannulated at the thoracic epidural cavity of T5～6 were randomly divided into three groups(n=20):sham operation group(group s),in which the anterior descending branches of left coronary artery were threaded by suture but not tied;heart failure group(groupHF),in which the anterior descending branches of left coronary artery were ligated,0.9%normal sodium 0.5ml/kg was injected to epidural space twice a bay for 4 weeks separately in group S and group HF.TEB group(group TEB),in which 0.1%ropivacaine 0.5ml/kg was injected to epidural space twice a day for 4 weeks after the anterior descending branches of left coronary artery were ligated.After 4 weeks,blood dynamics were monitored directly by insertion through common carotid artery and femoral artery;the level of ANP and ET-1 were mensurated by radioimmunoassay(RIA)and the expression of AQP4 in the lung was quantified by reverse transcription-polymerase chain reaction(RT-PCR).Results:(1)At 4 weeks after ligated LAD,LVEDP was increased significantly and MAP、LVSP、?dp/dt max were decreased significantly in group HF compared with group S.Therapy of TEB was significantly decreased LVEDP and were increased significantly MAP、LVSP、?dp/dt max.(2)Therapy of TEB decreased the level of ANP、ET-1 and increased the expression of AQP4mRNA of the rabbit with heart failure models.Conclusion:Thoracic epidural block have exert anti-heart failure effects by improving blood dynamics parameters,decreased secretion of ANP、ET-1and increased the expression of AQP4mRNA in the lung of rabbits.